Gene discovery research perspectives: Recent Episodes
Horizon Discovery, a PerkinElmer company
Engaging conversations with genome engineering and modulation experts on various topics from RNAi to CRISPR and emerging gene editing technologies to women and diversity in STEM.
As part of Horizon's The CRISPR Journal miniseries on CRISPR Functional Genomics, Ryan Donnelly discusses a user-friendly guide to streamlining CRISPR knockout workflows in cell lines including guide design, delivery and strategy, as well as tips for avoiding common pitfalls.
In this episode of Horizon's The CRISPR Journal miniseries on CRISPR Functional Genomics, Steve Smith, Product Manager with Horizon Discovery, reviews the latest tools in the CRISPR toolbox for gene modulation, activation and suppression.
As part of Horizon's The CRISPR Journal miniseries on CRISPR Functional Genomics, Jennifer Harbottle, Senior R&D Scientist with Horizon, discusses research and resources in the field of base editing.
As part of the CRISPR Functional Genomics Miniseries with The CRISPR Journal this podcast is a discussion about best practices when it comes to running CRISPR screens in primary cells. Enjoy this nice discussion with Nicola McCarthy, Horizon’s Screening Business Unit Manager, and Kevin Davies the executive editor of the CRISPR Journal.
In this podcast, Alan Dickson, Ph.D., Professor of Biology at the Manchester Institute of Biotechnology, University of Manchester talks about the evolution of the CHO Cell line from isolation to workhorse of the biomanufacturing industry, to gene edited knockout variants. An explanation is given why CHO cells have been so successful and how this success continues to be improved upon for manufacture of emerging therapeutics.
Professor Dickson discusses his group’s long trajectory in bioprocessing research, specifically the Chinese Hamster Ovary (CHO) cell line. Today CHO cells are the work horse for the development and manufacturing of biopharmaceutical products, but back in 1957 when Puck managed to isolate and keep in culture the first CHO-K1 cells, nobody could have anticipated this success.
Alan Dickson, Ph.D.
Professor of Biology at the Manchester Institute of Biotechnology
Professor Dickson is a molecular cell biologist who considers cells as factories that manufacture life-changing medicines, including protein-based biopharmaceuticals, viral vectors and modified cells. Working with industrial and academic collaborators, key advances have been developed by Dickson towards the understanding of how apoptosis, QC mechanisms and genome localization of recombinant genes can influence the efficiency of CHO cell systems. His laboratory use an application of ‘omics approaches to provide molecular profiling of CHO cells and how such information could be used to direct cell engineering towards improved manufacturing processes. Professor Dickson has been recognised with the Peter Dunnill award in 2017. In addition to his research, Alan teaches at Undergraduate and Postgraduate level and has held several senior administrative roles at the University of Manchester.