Biochemistry Class Notebook: Recent Episodes
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Post comments, criticisms and questions you may have in Biochemistry to help this blog develop. Use this as often as you need. The more we communicate...the more you learn.
This material assumes an advanced understanding of biology and chemistry as all of these videos are created after the actual lecture presentations on the topics. Send me your comments!!
The basic conception of a subject now known as Subatomic Particle physics dates back to 500 BC when the Greek philosopher Leucippus and his pupil Democritus suggested that matter consists of small, indivisible particles, which they called atoms. For more than 2000 years after this, the notion of atoms lay in obscurity. For quite a long time, people believed that all matter consisted of four elements: earth, fire, air, and water. We now know that atoms do exist, and that some particles smaller than atoms also exist.
We now know that atoms are not indivisible , since they contain protons, neutrons, electrons and other subatomic particles. However, other than this mistake Dalton's ideas are essentially correct in his Atomic Theory.
Matter is anything that has mass and takes up space. Matter has different states and their discovery and characterization employed the scientific method.
Significant Figures or Digits are the amount of digits that are accurate in a calculation. This is determined by how many digits there are in the numbers of the calculation.
Rising from the ashes...not to be defeated! Students, check out my introduction to chemistry for the student in the process technology department. I hope this will be helpful!
Introductory General Chemistry
Marion Carroll | Homework Paradise
Since leaving the classroom I have been longing to help students academically with their questions and concerns in basic chemistry. biology and biochemistry. Homework Paradise might be a way to do that. Give this a try. This portal is new to me so I will see how this goes and if it is useful in providing you with the help you seek. If so, I will begin promoting myself and you can help by submitting your own review. Thanks. Good luck!
Dr. Carroll
That's right. All done for another summer of kicking back, relaxin', readin', writing and some overall reflectin'. Things are looking up since the last time summer came around. Hope you enjoy yours in spite the challenges. God will bless in due time.
I welcome your comments here after or before your class. Suggestions for topics and clarification may be and are encouraged to be posted here. You can be anonymous or you may identify yourself. I especially would like to see comment strings, that is, what you think about what someone else has said. Come here often to review material covered in lecture.
Much of chapter 1 is a review of material you should have seen before with respect to thermodynamics and Gibbs Free Energy.
Chapter 1 contains a discussion of thermodynamics. Your comments are always welcomed. Enthalpy, entropy and free energy changes lie underneath all biochemical reactions driving them to completion and initiating the next reaction. Carefully read your chapter and search for other sources to reinforce these concepts.
So here's a little description of what I believe explains the difference between
Δ G and Δ G0.
Gibb's Free Energy
Here is a quick review of pH, pK and pI that will be important when we begin Chapter 4: amino acids.Amino Acid Titration.
For Window Media Players
Here is a summary of some important details concerning amino acid structure and properties. You should also be able to download theses videos for free from the iTunes music store if you have an account. You will find it in the Podcast section under "Biochemistry Class Notebook".
Stevie...what more can I say.
After discussing amino acids, protein primary, secondary and tertiary structure, we will discuss protein structure and function using hemoglobin: the classical allosteric protein.
Here is the first of 2 installments on our Enzyme Kinetics lectures. Use this for a quick review of the terms KM, VMAX, Kcat, KS and enzyme efficiency. More description of the Linweaver-Burke and inhibition will follow in the 2nd installment.
Hope you listen in on this description of the 3 common enzyme inhibition profiles, it may prove helpful on Exam III.
(click the title to play or right click it to download and play in Quicktime)
The first experiment in Biochemistry lab this coming week is the Spectrophotometry of Riboflavin. Take a look at the Spectrophotometry of Riboflavin, Part I before coming to class.
If you're having trouble downloading and viewing the link on the title then this link will redirect you to the source file and you should be able to hear and see it there.
(click the title to play or right click to download and play in Quicktime)
The first experiment in Biochemistry lab this coming week is the Spectrophotometry of Riboflavin. Take a look at the Spectrophotometry of Riboflavin, Part II video before class.
If you're having trouble downloading and viewing the link on the title then this link will redirect you to the source file and you should be able to hear and see it there.
Read your manual and do your calculations before class. Let me know if you have trouble viewing the Quicktime movie.
The amino acid titration experiment is described in this 20 minute video. CLICK the title to download and play a windows media file (not very good). Here you can download and play the quicktime file or get it from iTunes. Finally to accommodate all of you you may be able to view this video below. Go through it before taking the bonus quiz.
In the Experiment IV video will take a stock solution of BSA to generate a standard curve to use to determine the concentration of an unknown concentration of BSA. BSA is a standard size protein and is used regularly as a control to compare and determine concentrations of many different types of pure proteins. Can you tell me what BSA stands for?
Experiment VI and part of Experiment VII are described in this video. There is forthcoming the actual loading, running, staining and destaining of the gel before the final so stay tuned. You can access this video from iTunes or click the title here and view it or download it from my archives if having trouble viewing here. It may be necessary to wait until the video completes buffering to get a smooth view.